- Jeffery W. Carroll, Interim Chief of Police, January 2026-present
- Pamela Smith, July 2023-Dec. 2025
- Ashan Benedict (Interim), May-July 2023
- Robert J. Contee III, January 2021-May 2023
- Peter Newsham, September 2016-Dec. 2020
- Cathy L. Lanier, January 2007 - Sept. 2016
- Charles H. Ramsey, April 1998 - Dec. 2006
- Sonya Proctor (Interim), Nov. 1997 - April 1998
- Larry Soulsby, July 1995 - Nov. 1997
- Fred Thomas, December 1992 - July 1995
- Issac Fulwood, July 1989 - Sept. 1992
- Maurice T. Turner, Jr., July 1981 - July 1989
- Burtell M. Jefferson, January 1978 - June 1981
- Maurice J. Cullinane, December 1974 - Jan. 1978
- Jerry V. Wilson, August 1969 - Sept. 1974
- John B. Layton, December 1964 - July 1969
- Robert V. Murray, December 1951 - Dec. 1964
- Robert J. Barrett, July 1947 - Nov. 1951
- Harvey G. Callahan, February 1946 - June 1947
- Edward J. Kelly, November 1941 - Feb. 1946
- Ernest W. Brown, October 1932 - Nov. 1941
- Pelham D. Glassford, November 1931 - Oct. 1932
- Henry G. Pratt, April 1929 - Nov. 1931
- Edwin B. Hesse, October 1925 - April 1929
- Daniel Sullivan, February 1922 - Oct. 1925
- Harry L. Gessford, April 1920 - Dec. 1921
- Raymond W. Pullman, April 1915 - Feb. 1920
- Richard Sylvester, July 1898 - April 1915
- William G. Moore, December 1886 - July 1898
- Samuel H. Walker, July 1886 - Dec. 1886
- William M. Dye, April 1883 - June 1886
- William G. Brock, December 1879 - April 1883
- Thomas P. Morgan, February 1878 - Nov. 1879
- A.C. Richards, December 1864 - Jan. 1878
- William B. Webb, September 1861 - Nov. 1864